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KMID : 0613820210310070617
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Journal of Life Science
2021 Volume.31 No. 7 p.617 ~ p.625
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Nutritional Analyses and Antioxidant Activity of Apple Pomace
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Kim Ji-Eun
Shin Ji-Young
Yang Ji-Young
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Abstract
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To enhance the applications of apple pomace, which is a by-product of apple, this study analyzed the nutritional components, ursolic acid content, and antioxidant activity of different solvent (distilled water, fermented alcohol, and methanol) extracts. The samples included hot air-dried and freeze-dried apple pomace. The moisture, protein, fat, ash, and total dietary fiber contents of hot air-dried apple pomace were 3.2%, 3.9%, 2.4%, 2.0%, and 28.5%, respectively, and those of freeze-dried apple pomace were 8.2%, 3.4%, 2.4%, 1.8%, and 33.0%, respectively. Ursolic acid was not detected in the distilled water extract of either sample. However, in hot air-dried apple pomace, the methanol extract was 1,753.32 μg/ml, and the fermented alcohol extract was 1,532.94 μg/ml. In freeze-dried apple pomace, the methanol extract was 1,407.04 μg/ml, and the fermented alcohol extract was 1,221.81 μg/ml. The total polyphenol and flavonoid contents were 306.7 μg/ml and 950.1 μg/ml, respectively in methanol extracts of hot air-dried apple pomace and 277.6 μg/ml and 925.0 μg/ml, respectively in methanol extracts of freeze-dried apple pomace. 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities of hot air-dried apple pomace were 73.3% in methanol extract and 59.4% in fermented alcohol extract, and those of freeze-dried apple pomace were 76.1% in methanol extract and 66.0% in fermented alcohol extract. Both samples had the lowest antioxidant activity in distilled water extracts. Similar to DPPH radical scavenging activity, both samples showed increasing 2,2'-azino-bis(3-ethylbenzothiazoline-6- sulfonic acid) (ABTS) radical scavenging activity in the order of methanol, fermented alcohol, and distilled water. All samples had stronger reducing power than ascorbic acid (311.5 μg/ ml) as a positive control.
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KEYWORD
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Antioxidant activity, apple pomace, by-product, nutritional component, ursolic acid
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